Part 1:the basic principle of gene cloning
1.why gene cloning and DNA analysis are important
2.Vehicles for gene cloning: plasmids and bacteriophages
3.Purification of DNA form living cells
4.Manipulation of Purified DNA
5.Introduction of DNA into living cells
6.cloning vectors for E.coli
7.Cloning vectors for Eukaryotes
8.How to Obtain a clone of specific gene
9.The polymerase chain reaction

Part 2:The appplications of gene cloning and DNA analysis in research
10.Studying Gene Location and Structure
11.Studying Gene Expression and Function
12.Studying Gene Genomes

Part 3:the applications of gene cloning and DNA analysis in biotechnology
13 production of protein from cloned genes
14 gene cloning and DNA analysis in medicine
15 gene cloning and DNA analysis in Agriculture
16 gene cloning and DNA analysis in Forensic science

[ 本帖最后由 canadasnail 于 2006-7-5 10:39 编辑 ]

1pcr

1建立一个pcr实验室
2pcr残留污染的处理：一种酶学策略
3高保真pcr酶
4pcr的最优化和常见问题解析
5富含GC片段的PCR扩增
6精确扩增大片段的技巧
7pcr引物设计
8pcr扩增DNA的非放射性循环测序

2 样品的制备

9 DNA的纯化
10 RNA的纯化
11激光捕获微分离技术原位定位基因表达
12克服pcr受抑制的策略

3 RT-pcr的方法

13相对定量pt-pcr 和竞争定量rt-pcr内对照的使用
14四种实时pcr检测系统的比较：bio-rad I-cycler ,AbI 7700,Riche lightcycler ,the cepheid smartcycler
15定量pcr的新技术
16RNA 的扩增，用镁激活热稳定DNA聚合DNA扩增

以上是我最近阅读图书内容概要，如有什么需要讨论的，请跟贴。。。

RT-PCR跟NORTHERN的区别是什么？

不错的

ggooooodddd