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photon and Roman consuls
Scientists use two-photon absorption fluorescence to recover written information on Roman relics on Roman relics and solve a 2,000-year-old mystery.
When people think of archaeology, they often conjure images of adventurers such as Indiana Jones, who spend most of their time searching for mysterious ancient objects. We forget that the main objective in archaeology is to uncover the history hidden behind the objects, in the hope that they can help us understand a small fraction of the immense puzzle that is the past. Artifacts typically make a long journey from archaeological site to museum show case—with many stops along the way at high-tech research facilities.
In a discipline known as archaeometry, scientists use a number of chemical and physical procedures to obtain information from an archaeological artifact. Probably the most well-known strategy is the use of the radioactive isotope carbon-14 (14C) to date biological samples.
For non-organic objects, more indirect techniques may be used to allocate them to a particular historical context, such as studying the geological substrate where the piece was found or even the artistic style of the piece. Whenever possible, however, scientists opt for more direct techniques that gather qualitative and quantitative information about the chemical composition of the object.
With this information, researchers can trace the evolution of certain fabrication technologies through history. This information not only helps them to date archaeological objects, it enables them to identify the fabrication place and sources of raw material, revealing details about ancient trade and provenance. Analysis of chemical compounds in archaeology has been routinely based on ion beam and X-ray techniques.
Photonics enters the field of archaeometry mainly through the use of spectroscopic techniques, such as Raman spectroscopy, laser-induced fluorescence (LIF) and laser-induced breakdown spectroscopy (LIBS). These technologies allow for the fast, in situ identification of the chemical composition of a great variety of objects.
Researchers are also starting to use photonics for imaging within archaeology. For example, laser scan surveys can detect irregularities on a surface with important archaeological significance, uncovering features that are invisible to the naked eye. It is also used in cases where the topography of the sample is required but contact with the surface is not recommended. Examples of this are the scanning of carved rocks in the Paleolithic site of Cap Blanc, France, or the Stonehenge Scanning project.
Our archaeological problem
In our case, we were looking for a way to uncover information painted on the surface of a wine amphora that could help determine the foundation date of the Roman village of Iesso, which is nowadays the city of Guissona in Catalonia in northeast Spain. Archaeologists from the Institute of Classical Archaeology of Catalonia (ICAC) found this amphora among others near the city walls.
The place and disposition of the amphorae led the archaeologists to conclude that the objects were brought by the first Roman Legions that arrived on Iesso, and that the wine was consumed during a ritual celebration related to the wall building and the city foundation. As was usual at that time, the amphora had written on its neck the consular date—which is the name (initials) of the two Roman consuls ruling in the year of production. This was a signature of good quality wine.
Because the names and time frames for all the Roman consuls are known, this finding would allow us to date the foundation of the Roman city of Iesso. Unfortunately, at the moment of the discovery, the paint used to write the consular date was barely readable. In addition, since its discovery, natural photobleaching had taken its toll, and the archaeologists’ initial method for reading the inscription (humidifying the painted area with a water solution) had remarkably further deteriorated the quality of the paint. Using this method, the archaeologists were able to identify only the next group of letters:
Q
•
FAB
•
………..
•
COS
The first letters are the initials for Quintus Fabius, the first consul, while the last three letters signify Consolibus, a Latin word meaning “being consuls.” The name of the second consul should have been located at the space between these two groups of letters (i.e., the dotted line). However, this area was considerably damaged and most of the paint had disappeared, making it impossible to decipher the second consul name. Unfortunately, the knowledge of the first consul is not enough, since, during the same period, two different consuls owned the same name.
To uncover the missing name, we needed a detection technique that was able to detect and map small amounts of paint or some of its components (pigments or agglutinant). After examining the possible laser techniques, we discarded the use of LIBS and Raman spectroscopy. In the first case, the semidestructive nature of LIBS could damage the sample. In the second, the Raman signal generated by such a small amount of paint would be hardly distinguishable from the background signal generated by the pottery.
We decided that a technique based on fluorescence appeared to be the best option. Fluorescence from the paint is usually generated by the agglutinant, while the pigment, which is generally composed of metallic elements, inhibits it. This characteristic was quite convenient for our purposes; it helped us to identify previously painted areas where the pigment had disappeared but the agglutinant has persisted.
The amphora has been preserved in a dark area. Thus, an obvious requirement of our experiment was to avoid, at any price, laser-induced photodamage. This immediately prevented us from using high-energy photons for exciting the fluorescence. Unfortunately, it is known that the agglutinant used by the Romans (casein or albumin) fluoresces in the visible. This would require green-to-UV excitation sources, increasing the risk for photodamage. An alternative that was available in our lab was the use of femtosecond laser pulses to produce twophoton excitation fluorescence (TPEF), which allowed us to use near-infrared light as the excitation source, thereby greatly reducing the probability of producing photodamage.
Two-photon fluorescence imaging
The best known use for the non-damaging properties of TPEF is in nonlinear microscopy for biological applications.
Nonlinear microscopy uses infrared light to help ensure cell viability and is the technique of choice for imaging in vivoover extended observation periods. Our experimental set-up, shown in the figure below, reproduces a typical nonlinear microscope scheme, except for the fact that, for our purposes, high resolution was not required. In fact, the beam of a titanium: sapphire mode-locked laser was gently focused on the painted area on the amphora. The fluorescence signal was captured by a lens and directed through a hot mirror to the detection system, which was composed of a photomultiplier tube and a lock-in amplifier; this increased the sensitivity of the detection system. Two translation stages supporting the amphora composed the scanning system. Previous experiments on amphora fragments found in the same archaeological site allowed us to test our system and determine the photodamage threshold, optimal focusing and scanning parameters. These preliminary experiments also showed that the measured fluorescence was generated only from painted areas.
After these experiments, we scanned the painted area of the amphora, shown in the bottom of the figure on p. 23. In order to adapt to the amphora’s curvature and maximize the excitation of fluorescence, we divided the painted area into three regions, and scanned each region at three different focusing positions. The scanning of one zone was performed in 100 min in complete darkness.
The fluorescence image is shown in the inset image of the figure below. At first glance, the fragment may seem to show little evidence of any writing. However, as we previously mentioned, two vital pieces of information had to be considered. First, the generated fluorescence corresponded to regions where agglutinant was present, and, second, regions that contain pigment in fact inhibit the fluorescence.
In our scanned image, this resulted in large dark areas.
To fully recover the information on the amphora, we then performed a processing imaging technique based on histogram equalization that took these features into account.
[ 本帖最后由 kevinliu6883 于 2007-10-4 02:45 编辑 ] |
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