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[生化] 请教两道生化题,非常感谢!! [复制链接]

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发表于 2007-10-4 09:07:31 |显示全部楼层
请教两道生化题:

1、A plasmid that has two HindIII sites and two EcoRI sites, was cut with both of the enzymes simultanously for 5hrs at 37.C. The analysis of the restriction enzyme digestion was made on a 1% agarose gel. How many bands would one expect to see on the gel?
a 3       b 4         c 5         d 16          e No bands. Only smears

2、A viral genomic DNA that has two HindIII sites and two EcoRI sites, was cut with both of the enzymes simultanously for 5hrs at 37.C. The analysis of the restriction enzyme digestion was made on a 1% agarose gel. How many bands would one expect to see on the gel?
a 3       b 4         c 5         d 16          e No bands. Only smears

请教解题思路,非常谢谢!!

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发表于 2007-10-5 05:51:25 |显示全部楼层
The answer might be:
1. plasmid is circular DNA. so there are four strands on the gel
2. it depends on whether the viral DNA is circular or linear

[ 本帖最后由 hans19812002 于 2007-10-5 07:19 编辑 ]
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发表于 2007-10-5 08:40:33 |显示全部楼层
谢谢楼上,我明白了

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发表于 2007-10-5 11:02:25 |显示全部楼层
Viral genomic DNA  shouldn't be circular, maybe I'm wrong, try to find reference

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发表于 2007-10-6 04:10:17 |显示全部楼层

回复 #4 AA_workout 的帖子

In fact, genomic DNA of Phage lambda is circulized in bactiera
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发表于 2007-10-6 15:18:24 |显示全部楼层

回复 #5 hans19812002 的帖子

哈哈,lambda,我正想说的~不过感觉题目这么出,就算默认了
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RE: 请教两道生化题,非常感谢!! [修改]

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请教两道生化题,非常感谢!!
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