我现在也在和cornell的一个教授联系,因为我有一个师姐现在在他那里做,而且她听说她教授在找做我这个方向的学生,所以我就给他发了第一封信。我们主要是讨论学术问题,下面是我们的EMAIL:
On Nov 1, 2007, at 6:04 AM, yaoy@ualberta.ca wrote:
"Dear Dr.Jin,
I'm a undergrad student in University of Alberta. Currently i study on Enhancement of cytotoxicty by binding BH3 with Gold particle/Magnetic particle on MCF-7 Breast cancer cell line, but we encounter a problem, active site of BH3 is blocked by the particles, it decreases the specificity targeting, and cytotoxicity. So i wanna know how do your group solve this problem in terms of avoid blocking the active site of your desired antibody.Since your group currently proceed synthesis of iron oxide nanoparticles coated with antibody against transferrin receptor,which elicit transferrin receptor-mediated transcytosis of the particles into tissue.Or can you give some idea to try?
这是他给我回的
"several ways you can try. Particles can be coated with Protein A/G first, and then antibody is captured by them. Or reduce disulfides between heavy chains and use exposed cys for coupling. In our case, we often produce antibodies with Cys at the C-terminal to be used for coupling.
接下来我又给他发了一下我了一下MY experiment design,问他用我这种方法有什么问题吗?问题出在什么地方?但是他现在还没有回